유치 줄기세포에 대한 다양한 규산칼슘계 재료의 세포독성
Cytotoxicity of Various Calcium Silicate-based Materials with Stem Cells from Deciduous Teeth
윤지혜, 유용욱, 안은숙, 이준, 안소연,
소속 상세정보
윤지혜 ( Yun Ji-Hye ) - Wonkwang University School of Dentistry Department of Pediatric Dentistry
유용욱 ( You Yong-Ouk ) - Wonkwang University School of Dentistry Department of Oral Biochemistry
안은숙 ( Ahn Eun-Suk ) - Kyungbok University Department of Dental Hygiene
이준 ( Lee Jun ) - Wonkwang University School of Dentistry Department of Oral and Maxillofacial Surgery
안소연 ( An So-Youn ) - Wonkwang University School of Dentistry Department of Pediatric Dentistry
Abstract
The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro MTA® (RM), EZ-SealTM (EZ) and ENDOCEM Zr® (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ p( = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.
키워드
Cytotoxicity; Calcium silicate; Mineral trioxide aggregate; Deciduous tooth; Stem cells
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